Copy this page into your own repository and fill it in as you go through the course. Keep each section short: a figure and 2–4 sentences.
Gene ID and name: Gene family / predicted function:
1. Expression in Busia (Day 2)¶
Fold change and adjusted p-value in each contrast (selected vs parent, parent vs Kisumu). Volcano plot or bar plot.
2. Replication in other studies (Day 2)¶
AnoExpress: in how many experiments is it overexpressed? In which species and countries?
3. Genomic variation in wild populations (Day 3)¶
CNV frequencies and missense SNPs in Ugandan and Kenyan cohorts.
4. Selection (Day 3)¶
Does it lie within a selection-atlas signal in Busia or neighbouring cohorts? Which interval (focus/span1/span2)?
5. Assay design (Day 4)¶
Your best primer pair and/or probe. Why did you choose it? Does it avoid known SNPs?
6. Field evidence (Day 4)¶
Is the gene, or a linked marker on Ag-vampIR, associated with surviving deltamethrin in Siaya?
7. Verdict¶
How strong is the evidence overall? What experiment would you do next?